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Kupffer cells were isolated from normal and A-hypervitaminotic rats, and their vitamin A content and retinol esterifying activity were assayed in order to examine whether the Kupffer cells actually participate in the storage of vitamin A. The intrahepatic distribution of vitamin A was also studied using frozen sections of fresh and formalin-fixed livers by means of the fluorescence microscope. Both quantitative and qualitative data indicate that Kupffer cells are not the main site of vitamin A storage. The fluorescence of vitamin A and its reactivity to SbCl 3  decreased drastically after formalin treatment.

THE VITAMIN A CONTENT AND RETINOL ESTERIFYING ACTIVITY OF A KUPFFER CELL FRACTION OF RAT LIVER