THE VITAMIN A CONTENT AND RETINOL ESTERIFYING ACTIVITY OF A KUPFFER CELL FRACTION OF RAT LIVER | Zendy

Samuel H. Hori | Zendy; Takako Kitamura | Zendy

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THE VITAMIN A CONTENT AND RETINOL ESTERIFYING ACTIVITY OF A KUPFFER CELL FRACTION OF RAT LIVER

Author(s) -

Samuel H. Hori,

Takako Kitamura

Publication year - 1972

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/20.10.811

Subject(s) - vitamin , retinol , chemistry , kupffer cell , fluorescence microscope , medicine , endocrinology , biology , biochemistry , fluorescence , physics , quantum mechanics

Kupffer cells were isolated from normal and A-hypervitaminotic rats, and their vitamin A content and retinol esterifying activity were assayed in order to examine whether the Kupffer cells actually participate in the storage of vitamin A. The intrahepatic distribution of vitamin A was also studied using frozen sections of fresh and formalin-fixed livers by means of the fluorescence microscope. Both quantitative and qualitative data indicate that Kupffer cells are not the main site of vitamin A storage. The fluorescence of vitamin A and its reactivity to SbCl 3 decreased drastically after formalin treatment.

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