HISTOCHEMICAL DEMONSTRATION OF β-d-GLUCURONIDASE WITH A SYNTHETIC SUBSTRATE | Zendy

Arnold M. Seligman | Zendy; K. C. Tsou | Zendy; S. H. Rutenburg | Zendy; Richard B. Cohen | Zendy

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HISTOCHEMICAL DEMONSTRATION OF β-d-GLUCURONIDASE WITH A SYNTHETIC SUBSTRATE

Author(s) -

Arnold M. Seligman,

K. C. Tsou,

S. H. Rutenburg,

Richard B. Cohen

Publication year - 1954

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/2.3.209

Subject(s) - substrate (aquarium) , enzyme , gastrointestinal tract , uterus , thyroid , glucuronidase , epithelium , chemistry , spleen , biochemistry , biology , endocrinology , pathology , medicine , immunology , ecology

The synthesis of a substrate, 6-bromo-2-naphthyl β-d-glucopyruronoside, is described, and the procedure for the histochemical demonstrations of β-d-glucuronidase activity and experiments to determine the reliability of the results of localization of enzymatic activity are given. The results in normal tissues of the rat are presented. Greatest concentration of enzymatic activity was seen in liver, spleen, epithelium of gastrointestinal tract, uterus, thyroid, and in the white matter of the nervous system.

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