ULTRASTRUCTURAL LOCALIZATION OF CONTRACTILE PROTEIN (THROMBOSTHENIN) IN HUMAN PLATELETS USING AN UNLABELED ANTIBODY-PEROXIDASE STAINING TECHNIQUE | Zendy

François M. Booyse | Zendy; Dorothea Zschocke | Zendy; Max E. Rafelson | Zendy; Ludwig A. Sternberger | Zendy

Open Access

ULTRASTRUCTURAL LOCALIZATION OF CONTRACTILE PROTEIN (THROMBOSTHENIN) IN HUMAN PLATELETS USING AN UNLABELED ANTIBODY-PEROXIDASE STAINING TECHNIQUE

Author(s) -

François M. Booyse,

Dorothea Zschocke,

Max E. Rafelson,

Ludwig A. Sternberger

Publication year - 1971

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/19.9.540

Subject(s) - staining , horseradish peroxidase , ultrastructure , platelet , immunogold labelling , peroxidase , antiserum , antibody , immunocytochemistry , microbiology and biotechnology , pronase , chemistry , membrane , cytoplasm , primary and secondary antibodies , immunohistochemistry , biochemistry , biology , trypsin , enzyme , anatomy , immunology , endocrinology , genetics

Soluble horseradish peroxidase-antihorseradish peroxidase (rabbit) complex was used to localize the actomyosin-like contractile protein, thrombosthenin, in both intact human platelets (Epon-embedded) and ultrathin sections (methacrylate-embedded). Antibody staining of ultrathin sections showed the presence of membrane-associated and cytoplasmic thrombosthenin. Antibody staining of intact cells showed that membrane-associated thrombosthenin was localized, at least in part, in the exterior, "fluffy" coat of the platelet. Even after prolonged fixation and/or incubation with the various antisera, we were unable to demonstrate antibody penetration of intact, fixed platelets. Brief treatment with Pronase removed the surface-localized proteinaceous material and completely abolished all antibody staining.

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