GLUCOSE OXIDASE AS AN ANTIGEN MARKER FOR LIGHT AND ELECTRON MICROSCOPIC STUDIES
Author(s) -
W. D. Kuhlmann,
Stratis Avraméas
Publication year - 1971
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/19.6.361
Subject(s) - glucose oxidase , galactose oxidase , galactose , hydrogen peroxide , aspergillus niger , chemistry , oxidase test , peroxidase , biochemistry , staining , specific antibody , antibody , enzyme , microbiology and biotechnology , biology , immunology , genetics
A new staining procedure for glucose oxidase at both the light and the electron microscopic level has been developed. The procedure was equally effective for the detection of galactose oxidase. It appears that the method can be applied to all oxidoreductases which catalyze the production of hydrogen peroxide. Aspergillus niger glucose oxidase and Dacytlium dendroides galactose oxidase were used to immunize rabbits and mice in order to trace the distribution of specific antibodies in the immunocytes of the popliteal lymph nodes.
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