VISUALIZATION IN FREEZE-DRIED TISSUE SECTIONS OF STRUCTURES TO BE STUDIED BY QUANTITATIVE HISTOCHEMISTRY | Zendy

Bo Hellman | Zendy; Åke Lernmark | Zendy

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VISUALIZATION IN FREEZE-DRIED TISSUE SECTIONS OF STRUCTURES TO BE STUDIED BY QUANTITATIVE HISTOCHEMISTRY

Author(s) -

Bo Hellman,

Åke Lernmark

Publication year - 1971

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/19.3.186

Subject(s) - isopentane , staining , microanalysis , immunohistochemistry , pathology , chemistry , limiting , biochemistry , medicine , mechanical engineering , organic chemistry , engineering , catalysis

The limiting factor for quantitative histochemistry employing freeze-dried tissue sections is the ability to identify morphologic structures. Satisfactory staining of freeze-dried tissue specimens intended for further microanalysis was achieved by using isopentane solutions of free dye bases. The practical details of this rapid histologic control are outlined and its usefulness is illustrated by measurements of fructose 1,6-diphosphate and insulin in microdissected specimens of pancreatic β cells.

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