AN AUTOMATIC METHOD FOR VIABILITY ASSAY OF CULTURED CELLS | Zendy

Regina C. O'Brien | Zendy; PHYLLIS GOTTLIEB-ROSENKRANTZ | Zendy

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AN AUTOMATIC METHOD FOR VIABILITY ASSAY OF CULTURED CELLS

Author(s) -

Regina C. O'Brien,

PHYLLIS GOTTLIEB-ROSENKRANTZ

Publication year - 1970

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/18.8.581

Subject(s) - viability assay , trypan blue , cell counting , fluorescence , population , cell , absorption (acoustics) , microbiology and biotechnology , biology , chemistry , biophysics , optics , biochemistry , cell cycle , physics , medicine , environmental health

An automatic method for viability assay of a population of cells is described. Cell populations are stained with trypan blue (for dead cells) and fluorescein diacetate (for live cells). A rapid cell spectrophotometer measures changes in light intensity which occur when individual cells pass through the photometric field. Tallies of the number of decreases (absorption and scatter by dead cells) and the number of increases (fluorescence by live cells) in light intensity are recorded and viability percentages are calculated. These automatically determined viability percentages are compared with viability percentages determined by visual counting and classifying of cells from the same populations.

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