MICROPROBE ANALYSIS OF PROTARGOL STAIN DEPOSITION IN TWO PROTOZOA | Zendy

Ian S. Zagon | Zendy; Jiří Vávra | Zendy; I. M. Steele | Zendy

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MICROPROBE ANALYSIS OF PROTARGOL STAIN DEPOSITION IN TWO PROTOZOA

Author(s) -

Ian S. Zagon,

Jiří Vávra,

I. M. Steele

Publication year - 1970

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/18.8.559

Subject(s) - microprobe , stain , electron microprobe , staining , protozoa , ciliata , ciliate , chemistry , biology , mineralogy , chromatography , botany , paleontology , genetics

Protargol-stained specimens of two protozoans, Carchesium polypinum (Ciliata, Peritrichida) and Gregarina rhyparobiae (Sporozoa, Eugregarinida), were examined by an electron microprobe analyzer in order to determine the elemental composition of the stain deposit. Limited penetration of the microprobe beam presumably revealed only the composition of the stain deposits close to the surface of the examined protozoa. The data on this first analysis of Protargol staining revealed that element detection was negligible in the unstained preparations, but the stained specimens of both protozoans revealed a mass concentration ratio of 0.4 Cu:1 Ag:6.1 Au. There was no significant difference in the element ratio between the two protozoan species belonging to different classes and stained with slightly different stain procedures. Some aspects of Protargol mechanisms are discussed along with an interpretation of data and an indication of the feasibility of further examinations.

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