CONTRIBUTION TO THE CYTOSPECTROFLUOROMETRIC MEASUREMENT OF 5-HYDROXYTRYPTAMINE IN ENTEROCHROMAFFIN CELLS | Zendy

M Vialli | Zendy; G Prenna | Zendy

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CONTRIBUTION TO THE CYTOSPECTROFLUOROMETRIC MEASUREMENT OF 5-HYDROXYTRYPTAMINE IN ENTEROCHROMAFFIN CELLS

Author(s) -

M Vialli,

G Prenna

Publication year - 1969

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/17.5.321

Subject(s) - enterochromaffin cell , fluorescence , paraformaldehyde , chemistry , formaldehyde , absorption (acoustics) , analytical chemistry (journal) , aqueous solution , absorption spectroscopy , spectral line , excitation , chromatography , materials science , serotonin , optics , biochemistry , physics , receptor , organic chemistry , composite material , electrical engineering , astronomy , engineering

Cytofluorometry enables, under certain conditions, quantitative determination of the 5-hydroxytryptamine content of enterochromaffin cells. Preparations were obtained by: (1) fixation with 10% neutral formalin, (2) freeze-drying and exposure of the sections to formaldehyde released from aqueous formalin or paraformaldehyde and (3) Falck's method. For measurements the Leitz MPV microspectrophotometer and microspectrograph with a television oscilloscopic system were used. Absorption spectra, excitation spectra, emission spectra, photolysis and emission intensity recovery in cells were measured. The corrected data obtained included: absorption peak, 390 nm; excitation peak, 360 to 370 nm; and emission peak, 560 nm. Comparative observations were made on the fluorescence of the background.

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