A FLUORESCENT METHOD FOR THE DETECTION AND LOCALIZATION OF ZINC IN HUMAN GRANULOCYTES | Zendy

G. Smith | Zendy; Robert A. Jenkins | Zendy; JAMES F. GOUGH | Zendy

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A FLUORESCENT METHOD FOR THE DETECTION AND LOCALIZATION OF ZINC IN HUMAN GRANULOCYTES

Author(s) -

G. Smith,

Robert A. Jenkins,

JAMES F. GOUGH

Publication year - 1969

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/17.11.749

Subject(s) - zinc , dithizone , fluorescence , stain , chemistry , staining , fluorescent staining , magnesium , calcium , biochemistry , chromatography , biology , organic chemistry , physics , quantum mechanics , genetics

A new cytochemical method is presented for the fluorescent demonstration of zinc in human granulocytes using 8-hydroxyquinoline. This compound is known to form fluorescent complexes with magnesium, calcium and zinc. Specificity for zinc is accomplished by adjusting the staining solution to pH 8.0 with Michaelis universal buffer. The technique is simple, rapid and much more specific for zinc than the dithizone stain.

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