A METHOD FOR DIFFERENTIATING DOPAMINE FROM NORADRENALINE IN TISSUE SECTIONS BY MICROSPECTROFLUOROMETRY | Zendy

Anders Björklund | Zendy; Berndt Ehinger | Zendy; B. Falck | Zendy

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A METHOD FOR DIFFERENTIATING DOPAMINE FROM NORADRENALINE IN TISSUE SECTIONS BY MICROSPECTROFLUOROMETRY

Author(s) -

Anders Björklund,

Berndt Ehinger,

B. Falck

Publication year - 1968

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/16.4.263

Subject(s) - dopamine , fluorophore , isoquinoline , chemistry , fluorescence , formaldehyde , norepinephrine , biophysics , biochemistry , medicine , stereochemistry , biology , physics , optics

Fluorophores induced from noradrenaline and dopamine in tissue sections by treatment with formaldehyde can be separated by their different behavior upon HCl treatment. The noradrenaline fluorophore converts to a fully aromatic isoquinoline while the dopamine fluorophore remains in the form of a nonquinoidal dihydroisoquinoline. The two fluorophores have readly distinguishable excitation spectra. The procedure has been tested both in model systems and in tissue sections. The microspectrofluorometer used is described.

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