Transcriptional Changes in Cancer Cells Induced by Exposure to a Healing Method
Author(s) -
Beseme Sarah,
Bengston William,
Radin Dean,
Turner Michael,
McMichael John
Publication year - 2018
Publication title -
dose-response
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.502
H-Index - 36
ISSN - 1559-3258
DOI - 10.1177/1559325818782843
Subject(s) - reverse transcription polymerase chain reaction , inflammation , gene , wound healing , in vitro , gene expression , breast cancer , real time polymerase chain reaction , biology , cancer research , medicine , cancer , bioinformatics , immunology , biochemistry , genetics
Energy healing, or healing with intent, is a complementary and alternative medicine therapy reported to be beneficial with a wide variety of conditions. We are developing a delivery technology for a method previously tested in mouse models with solid tumors (the Bengston method) independent of the presence of a healer. The goal of this study was to assess whether stored or recorded energy has an impact on breast cancer cells in vitro, using energy-charged cotton and electromagnetic recording of healers practicing the method. Expression of genes involved in cancer and inflammation pathways was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Treatment of cells using energy-charged cotton resulted in statistically significant changes <1.5-fold. In cells exposed to an electromagnetic recording, 37 genes of 167 tested showed a >1.5-fold change when compared to the control, and 68 genes showing statistically significant fold changes. Two genes, ATP citrate lyase ( ACLY ) and interleukin 1β ( IL-1β ), were consistently downregulated at 4 and 24 hours of exposure to the recording, respectively, in 3 independent experiments. Both ACLY and IL-1β were also downregulated in cells exposed to a hands-on delivery of the method, suggesting these 2 genes as potential markers of the healing method.
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