Open AccessHigh-quality RNA improves sensitivity of SARS-CoV-2 detection by colorimetric RT-LAMP
Author(s) -
Marta Puigmulé,
Mònica Coll,
Alexandra PérezSerra,
Laura López,
Fernando Pico,
Nuria Neto,
Mònica Corona,
Mel Lina Pinsach-Abuin,
Carles Ferrer-Costa,
María Buxó,
Francesc-Xavier Queralt,
Ramón Brugada
Publication year - 2021
Publication title -
experimental biology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 146
eISSN - 1535-3702
pISSN - 1535-3699
DOI - 10.1177/15353702211054768
Subject(s) - reverse transcription loop mediated isothermal amplification , loop mediated isothermal amplification , rna , reverse transcriptase , reverse transcription polymerase chain reaction , gold standard (test) , biology , covid-19 , virology , microbiology and biotechnology , medicine , messenger rna , gene , dna , pathology , biochemistry , disease , infectious disease (medical specialty)
The global SARS-CoV-2 pandemic requires a rapid, reliable, and user-friendly diagnostic test to help control the spread of the virus. Reverse transcription and quantitative PCR (RT-qPCR) is currently the gold standard method for SARS-CoV-2 detection. Here, we develop a protocol based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and demonstrate increased sensitivity of this technique using fresh RNA extracts compared to RNA samples subjected to freezing/thawing cycles. We further compare RT-LAMP to RT-qPCR and demonstrate that the RT-LAMP approach has high sensitivity in fresh RNA extracts and can detect positive samples with Ct values between 8 and 35.