Open AccessThe Involvement of lncRNA HOTAIR/miR-130a-3p Axis in the Regulation of Voluntary Exercise on Cognition and Inflammation of Alzheimer’s Disease
Author(s) -
Jianxia Lü,
Lihua Liu,
Jin Chen,
Juan Zhi,
Jiabin Li,
Le Li,
Zhongli Jiang
Publication year - 2022
Publication title -
american journal of alzheimer's disease and other dementias
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.653
H-Index - 60
eISSN - 1938-2731
pISSN - 1533-3175
DOI - 10.1177/15333175221091424
Subject(s) - hotair , morris water navigation task , inflammation , cognition , disease , medicine , oncology , neuroscience , psychology , biology , downregulation and upregulation , long non coding rna , psychiatry , biochemistry , gene
Background Alzheimer’s disease (AD) is an age-related neurodegenerative disease and exercises might mitigate the progression of AD. This investigation aimed to manifest the potential mechanism of exercises in AD.Methods Morris water maze (MWM) test was conducted to evaluate the cognitive function in APP/PS1 mice. Quantitative real-time PCR was performed to detect the expression of HOTAIR and miR-130a-3p. The enzyme-linked immunosorbent assay was applied to appraise the concentration of IL-1β, IL-6, and TNF-α. A luciferase report experiment was implemented to substantiate the relationship between miR-130a-3p and HOTAIR.Results Exercises contributed to the elevated expression of HOTAIR. The findings of MWM implied HOTAIR inhibited the impacts of voluntary exercises on escape latency, distance moved, percentage of time spent in the target quadrant, platform crossing times, and inflammation. MiR-130a-3p mediated the function of HOTAIR on cognitive ability and inflammation.Conclusion HOTAIR participated in the regulation of exercises on AD by sponging miR-130a-3p.