Open AccessThe Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles
Author(s) -
Ghaleb A. Husseini,
Kim L. O’Neill,
William G. Pitt
Publication year - 2005
Publication title -
technology in cancer research and treatment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.754
H-Index - 63
eISSN - 1533-0346
pISSN - 1533-0338
DOI - 10.1177/153303460500400616
Subject(s) - propidium iodide , lysis , comet assay , doxorubicin , programmed cell death , microbiology and biotechnology , apoptosis , chemistry , poloxamer , dna damage , pathology , biology , medicine , dna , chemotherapy , biochemistry , surgery , organic chemistry , copolymer , polymer
This notes examines the mode of cell death of HL-60 cells exposed to 70 kHz and 1.3 W/cm 2 ultrasound in the presence of 1% Pluronic P105 and 1.67 μg/ml doxorubicin (Dox). The cells were ultrasonicated for 30, 60, and 120 minutes. They were then lysed, electrophorised, stained using propidium iodide, and their DNA profile captured using a fluorescent microscope. The gradual DNA damage observed and the comet tails captured after one and two hours of insonation suggest that the mode of cell killing is apoptosis.