PEPSIN DIGESTION OF VIRUS PARTICLES IN CANINE HEPATITIS USING EPON-EMBEDDED MATERIAL | Zendy

Kathleen F. Givan | Zendy; Charlotte Turnbull | Zendy; Anne-Marie Jézéquel | Zendy

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PEPSIN DIGESTION OF VIRUS PARTICLES IN CANINE HEPATITIS USING EPON-EMBEDDED MATERIAL

Author(s) -

Kathleen F. Givan,

Charlotte Turnbull,

Anne-Marie Jézéquel

Publication year - 1967

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/15.11.688

Subject(s) - paracrystalline , pepsin , virus , virology , inoculation , digestion (alchemy) , electron microscope , chemistry , biology , microbiology and biotechnology , chromatography , enzyme , immunology , biochemistry , physics , optics , crystallography

Sixteen-week-old dogs were inoculated with 20,000 tissue culture doses (TCD 50 ) of infectious canine hepatitis virus. Four days after inoculation, liver cells examined in the electron microscope contained both virus particles and nonviral paracrystalline formations. Tissue culture cells also contained virus particles and nonviral paracrystalline formations 48 hr after inoculation with virus. When Epon-embedded sections were treated for 1 hr with 0.5% pepsin in 0.1 N HCl, a selective extraction of the viral coats and of the nonviral paracrystals was observed.

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