DETECTION OF HISTAMINE BY A NEW FLUORESCENT o-PHTHALALDEHYDE STAIN | Zendy

L Juhlin | Zendy; Walter B. Shelley | Zendy

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DETECTION OF HISTAMINE BY A NEW FLUORESCENT o-PHTHALALDEHYDE STAIN

Author(s) -

L Juhlin,

Walter B. Shelley

Publication year - 1966

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/14.7.525

Subject(s) - histamine , o phthalaldehyde , stain , pathology , chemistry , fluorescence , staining , fluorescence microscope , fluorescent staining , platelet , microbiology and biotechnology , biology , endocrinology , medicine , chromatography , immunology , mass spectrometry , derivatization , physics , quantum mechanics

A simple, rapid technique has been developed for the visualization of histamine in its cellular depots throughout the body. Based on the yellow fluorochrome formed by the interaction of o-phthalaldehyde and histamine, this procedure permits direct fluorescence microscopy of the "histaminocytes." Study of fresh blood and tissues from man and many animals revealed histamine its the mast cells, blood vessels, gastric mucosa, and in some species, platelets.

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