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A megakaryocyte cell series from rat bone marrow has been examined by the isotopic di-isopropyl fluorophosphate (DFP) method for esterases. After complete reaction with  32 P-DFP, the numbers of DFP-reacted molecules in individual cells have been determined by β track auto-radiography. Previous work has shown the percentage of organophosphate-sensitive sites in these cells which can be taken as active centers of acetylcholinesterase (AChase). Combining these data, the absolute numbers of organophosphate-sensitive esterase molecules and AChase molecules per cell were determined. Histograms show a narrow spread of values within each of four size classes from megakaryoblast to fully mature megakaryocyte, but, with means increasing 4-fold through this series, approximately in proportion to cell volume. A rat megakaryoblast has 2 x 10 6  AChase molecules, and a megakaryocyte (of 48-µ diameter) has 7.6 x 10 6  molecules. The apparent turnover number of the enzyme for intracellular reaction with substrate is calculated and compared with turnover numbers available for other AChases.

THE NUMBER OF ACETYLCHOLINESTERASE MOLECULES IN THE RAT MEGAKARYOCYTE