Open AccessEffects of intermittent negative pressure treatment on circulating vascular biomarkers in patients with intermittent claudication
Author(s) -
Henrik Hoel,
Erik Mulder Pettersen,
Lars Øivind Høiseth,
Iacob Mathiesen,
Arne Seternes,
Ingebjørg Seljeflot,
Jonny Hisdal
Publication year - 2021
Publication title -
vascular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.838
H-Index - 73
eISSN - 1477-0377
pISSN - 1358-863X
DOI - 10.1177/1358863x211007933
Subject(s) - medicine , von willebrand factor , intermittent claudication , asymmetric dimethylarginine , biomarker , endothelium , claudication , endothelial dysfunction , cardiology , endocrinology , gastroenterology , vascular disease , arginine , platelet , arterial disease , biochemistry , chemistry , amino acid
The aim of this study was to investigate the effects of lower extremity intermittent negative pressure (INP) treatment for 1 hour twice daily for 12 weeks, on circulating vascular biomarkers in patients with intermittent claudication. Patients were randomized to treatment with -40 mmHg INP (treatment group), or -10 mmHg INP (sham control group). Venous blood samples were collected at baseline and after 12 weeks, and concentrations of vascular adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), E-selectin, P-selectin, von Willebrand factor (vWF), l-arginine, asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA) were analyzed. A larger proportion of the patients in the treatment group (25/31) had a reduction in vWF levels after 12 weeks, compared to the sham control group (17/30) ( p = 0.043). Within the treatment group there was a significant mean (SEM) reduction in the concentration of vWF of -11% (4) ( p = 0.019), whereas there was no significant change in the levels of vWF in the sham control group (1% (6); p = 0.85). There were no significant differences in the change of any of the biomarker levels between the groups after 12 weeks of treatment. In conclusion, there were no differences in the change of the circulating levels of the measured biomarkers between the treatment group and the sham control group after 12 weeks of INP treatment. However, the observed changes in vWF might indicate a beneficial effect of INP treatment on endothelial activation and endothelial injury. Clinicaltrials.gov Identifier: NCT03640676 .