Open AccessA METHOD FOR THE SIMULTANEOUS DEMONSTRATION OF CHOLINE-CONTAINING PHOSPHOLIPIDS AND NEUTRAL LIPIDS IN TISSUE SECTIONS
Author(s) -
Curtis H. Bourgeois,
Barbara Burke Hubbard
Publication year - 1965
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/13.7.571
Subject(s) - chromatography , staining , oil red o , neutral red , chemistry , choline , phosphatidyl choline , biochemistry , phospholipid , biology , in vitro , genetics , cytotoxicity , membrane , adipogenesis
A modification of Baker's dichromate-acid hematein test combined with an Oil Red O counterstain is presented. Chromatographic analysis of the procedure clearly demonstrates the selective staining of choline-containing phospholipids (blue-grey) and neutral lipids (red).
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