SEPARATION AND RELATIVE QUANTITATION OF MOUSE PLASMA ESTERASES WITH DISC ELECTROPHORESIS
Author(s) -
Robert C. Allen,
R A Popp,
Dorothy James Moore
Publication year - 1965
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/13.4.249
Subject(s) - quantitative analysis (chemistry) , chromatography , electrophoresis , acrylamide , chemistry , substrate (aquarium) , biology , polymer , copolymer , ecology , organic chemistry
A technique for the qualitative and quantitative study of mouse plasma esterases using disc electrophoresis in acrylamide gel is described. This technique has been employed to study substrate specificity and quantitative relationships among various mouse strain and sex dependent esterases. Properties of acrylamide gels are discussed relative to their effect on the quantitative estimation of plasma esterases.
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