SOME ASPECTS OF THE SPECIFICITY OF FATTY ACID ESTERASES
Author(s) -
B.H.J. Hofstee
Publication year - 1964
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/12.9.700
Subject(s) - kinetic energy , interpretation (philosophy) , chemistry , substrate (aquarium) , group (periodic table) , substrate specificity , enzyme , hydrolase , peptide hydrolases , stereochemistry , biochemistry , organic chemistry , physics , biology , computer science , classical mechanics , ecology , programming language , protease
A comparison has been made of the meaning of the experimental kinetic constants V m , K M and V m /K M with respect to group transfer by a hydrolase proceeding either with or without obligatory intermediary transfer of the group to the enzyme. The results have been applied to the interpretation of kinetic data in a number of actual cases involving esterases whereby either several substrates have one of these experimental constants in common or the addition of a modifier has no effect on one of these constants with respect to a particular substrate.
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