ACID DEOXYRIBONUCLEASE IN RAT LIVER CELL NUCLEI ISOLATED IN THE PRESENCE OF CALCIUM IONS | Zendy

Karl F. Swingle | Zendy; Leonard J. Cole | Zendy

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ACID DEOXYRIBONUCLEASE IN RAT LIVER CELL NUCLEI ISOLATED IN THE PRESENCE OF CALCIUM IONS

Author(s) -

Karl F. Swingle,

Leonard J. Cole

Publication year - 1964

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/12.6.442

Subject(s) - calcium , deoxyribonuclease , isotonic , chemistry , cytosol , deoxyribonuclease i , isotopes of calcium , biochemistry , deoxyribonucleases , sucrose , enzyme , cell nucleus , cytoplasm , dna , medicine , organic chemistry , base sequence

Nuclear fractions centrifugally separated from rat liver homogenates in isotonic sucrose or in water, containing in each case 1.8 mM CaCl 2 , and washed repeatedly in the same media, retained a considerable amount of acid deoxyribonuclease activity. Similarly prepared nuclear fractions using calcium-free media lost nearly all of their deoxyribonuclease activity. This retention of activity in the presence of calcium was shown to be neither cosedimentation of unbroken cells or of lysosomes with the nuclear fraction nor precipitation of soluble deoxyribonuclease in the presence of calcium. The nuclear-bound enzyme was active under conditions shown not to remove it from the nuclei.

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