CYTOCHEMISTRY OF PARACOCCIDIOIDES BRASILIENSIS. I. CYTOCHEMISTRY OF CYTOPLASMIC POLYSACCHARIDES IN YEAST FORM CULTURES WITH LIGHT MICROSCOPE
Author(s) -
Luis M. Carbonell,
Haydée V. Castejón,
Ladislao Pollak
Publication year - 1964
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/12.6.413
Subject(s) - paracoccidioides brasiliensis , cytochemistry , polysaccharide , glycogen , cytoplasm , yeast , biochemistry , biology , acid phosphatase , staining , microbiology and biotechnology , enzyme , genetics
1. The histochemical distribution and localization of cytoplasmic polysaccharides are studied in the yeast form of P. brasiliensis. Glycogen and an acid mucopolysaccharide probably containing sulfate groups are demonstrated. These polysaccharides disappear after the sixth day of culture. The glycogen and the acid mucopolysaccharide are bound to protein. 2. Stains and enzyme treatments applied in sequence help demonstrate the site of different polysaccharides.
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