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HISTOCHEMICAL RECOGNITION OF CALCIUM OXALATE
Author(s) -
Philip Pizzolato
Publication year - 1964
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/12.5.333
Subject(s) - calcium oxalate , silver nitrate , oxalate , ethylene glycol , chemistry , ammonium oxalate , hydrogen peroxide , calcium , nuclear chemistry , calcium nitrate , calcium oxalate crystals , ammonium nitrate , ammonium , inorganic chemistry , nitrate , biochemistry , organic chemistry
Of the workers applying the von Kóssa test to calcium oxalate crystals in animal tissue, about half reported blackening in silver nitrate on exposure to light. We found that "pure" calcium oxalate crystals, produced in rats by the administration of ammonium oxalate or ethylene glycol, gave a negative von Kóssa reaction. These crystals, however, were blackened when treated with a solution of silver nitrate in concentrated hydrogen peroxide. Other von Kóssa negative salts, such as CaF 2 or CaSO 4 ·2H 2 O, do not respond to the H 2 O 2 and AgNO 3 test.

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