A SILVER STAIN FOR DEOXYRIBONUCLEIC ACID | Zendy

Roy Korson | Zendy

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A SILVER STAIN FOR DEOXYRIBONUCLEIC ACID

Author(s) -

Roy Korson

Publication year - 1964

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/12.12.875

Subject(s) - stain , trichloroacetic acid , chemistry , grocott's methenamine silver stain , citric acid , silver nitrate , chromatography , aqueous solution , deoxyribonuclease , perchloric acid , staining , extraction (chemistry) , biochemistry , dna , nuclear chemistry , pathology , inorganic chemistry , medicine

By hydrolyzing tissue sections with 1 M citric acid for 30 minutes at 60°C and subsequently treating the sections with methenamine silver for 60 minutes at 60°C (5% silver nitrate in 3% aqueous methenamine), a stain has been developed which is extremely intense and specific for nuclear deoxyribonucleic acid. This has been tested in a number of ways including deoxyribonuclease, trichloroacetic and perchloric acid extraction. The reaction has been successful with fresh frozen cryostat sections as well as with paraffin-embedded material. The stain is likewise satisfactory with tissue fixed in neutral formalin, Carnoy's solution, or formalin-phosphate-sucrose mixture. Because the silver precipitate is precisely localized it may be useful in visualizing chromatin with the electron microscope.

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