DEVELOPMENT OF A HISTOCHEMICAL METHOD FOR α-d-GALACTOSIDASE AND ITS DISTRIBUTION IN THE RAT | Zendy

Benito Monis | Zendy; K. C. Tsou | Zendy; Arnold M. Seligman | Zendy

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DEVELOPMENT OF A HISTOCHEMICAL METHOD FOR α-d-GALACTOSIDASE AND ITS DISTRIBUTION IN THE RAT

Author(s) -

Benito Monis,

K. C. Tsou,

Arnold M. Seligman

Publication year - 1963

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/11.5.653

Subject(s) - cytoplasm , frozen section procedure , incubation , chemistry , thyroid , kidney , distribution (mathematics) , biology , microbiology and biotechnology , biochemistry , endocrinology , pathology , medicine , mathematical analysis , mathematics

A histochemical method for the demonstration of α-d-galactosidase activity in formalinfixed, frozen sections was developed by the synthesis of 6-bromo-2-naphthyl-α-d-galactopyranoside. The histochemical procedure is similar to that developed for other glycosidases and utilizes the post-incubation coupling principle. A survey of the organs of the rat revealed the highest activity in the cytoplasm of epithelial cells of Brunner's glands of the intestine, distal segment of proximal tubules of the kidney, thyroid and parathyroid glands and very strikingly in heterophiles and megakaryocytes.

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