Book Reviews

451 ‘i’he Interpretation of Ultrastructure, Volume m. Edited l)y H. J. C. HARRIS, Academic Press, Inc., New York, 1962. 427 pp., chotinbound, $14.00. This volumnue is tine proceedings of a symposium of tine International Society for Cell Biology that was held in Bern, Switzerland, September, 1961. Like so nnany published synuposia it is regrettable that a year and a half has elapsed between the synupo)siunui and its publication. Included are 22 papers with references and discussion. Tine discussion in some cases is illuminating and tine inchuision of sanue in the publication is comuiniendable. Included in the presentation are papers concerned witin techniques of electron nunicroscopy-fixat ion, negative staining, enubedding nuedimu, anol qunantitation. There are papers on nuuclemur structure, clneniistry, and tine nnitotic apparatus. Lipoprotein membrane systems are discussed in thnree papers. Among tine renuaining presenutations are ones concerned with correlation of higint mind electron microscopy, embryonic differentiation, ultratexture of plant cell walls, annul naturmul cellulose stuidied by electromn microsOI)V and elect romn diffraction. ‘runere mire three papers (mat are of especial interest. to inistocinemists. Leduc and Bernhard discunss t heir experiences witiu formalin-fixed tissues for tine action of pepsin, trypsin, deoxyribonuiclease, munch ribonuclease mund the relative merits of tinree wmuter-soluble embedding nuedia. Tine tise of wmuter-soluble embedding media molds gremit pronuise for future applications of cytocinenuical tecinniques to electron microscopy . Holt discunsses the Problenis associated with tine use of conventional cytochensical nuetinods for enzynse locmuhization applied to electron microscopy. Like others Bolt found that. osmiuni tetroxidc fixation (Irastically reduces enzynuic activity, and that formalin preserves enzymic activity in addition to providing good tissue preservation for elect ruIn nunicroscopv Holt reconunuends a pinospinmute-bunifered formunahin. Ledunc and Bernhard foumnd a l)inosPinat e-i)ulffered fornuah in satisfactory amid also used mu i)mlri)iturate buffer. In discussing tine paper of Leolunc mund Bernhard, Holt pointed out that fornuahin reacts witin barbiturate yielding a