Open AccessThe Influence of Low Platelet Count on Whole Blood Aggregometry Assessed by Multiplate
Author(s) -
Trine Stissing,
Nadia Paarup Dridi,
Sisse Rye Ostrowski,
Louise Bochsen,
Pär I. Johansson
Publication year - 2011
Publication title -
clinical and applied thrombosis/hemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.643
H-Index - 53
eISSN - 1938-2723
pISSN - 1076-0296
DOI - 10.1177/1076029610397183
Subject(s) - platelet , whole blood , heparin , platelet aggregation , platelet rich plasma , medicine , platelet poor plasma , chemistry , platelet activation , pharmacology
The Multiplate, a whole blood (WB) platelet function test, has shown promising results identifying patients on antiplatelet therapy at increased risk of rethrombosis. In the present study, the influence of low platelet count on platelet aggregation was analyzed and compared with aggregation results in an artificial matrix, platelet-rich plasma (PRP). Heparinized and citrated blood was diluted with autologous plasma to platelet concentrations 200 to 25 × 10 9 /L in WB samples (n = 10) and 200 to 100 × 10 9 /L in PRP samples (n = 7). The platelet aggregation was investigated by the ADP-, ASPI-, COL-, and TRAP-test. The WB responses decreased at platelet concentration of ≤100 × 10 9 /L (all P < .03), except for heparin-TRAP (50 × 10 9 /L, P = .008) and citrate-ASPI (150 × 10 9 /L, P = .03). In general, WB samples demonstrated higher aggregation than PRP samples at platelet concentrations 200 to 100 × 10 9 /L ( P < .05). In conclusion, platelet concentration of <150 × 10 9 /L may influence Multiplate which should be considered in clinical settings. Furthermore, the findings emphasize the importance of evaluating haemostasis in its natural matrix, WB.