Detection of Brucella canis infection in dogs by blood culture and bacterial identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry | Zendy

Tanya Purvis | Zendy; Donna Krouse | Zendy; Dawn Miller | Zendy; Julia Livengood | Zendy; Nagaraja Thirumalapura | Zendy; Deepanker Tewari | Zendy

Open Access

Detection of Brucella canis infection in dogs by blood culture and bacterial identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Author(s) -

Tanya Purvis,

Donna Krouse,

Dawn Miller,

Julia Livengood,

Nagaraja Thirumalapura,

Deepanker Tewari

Publication year - 2017

Publication title -

journal of veterinary diagnostic investigation

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.529

H-Index - 78

eISSN - 1943-4936

pISSN - 1040-6387

DOI - 10.1177/1040638717704652

Subject(s) - brucella , canis , mass spectrometry , brucellosis , microbiology and biotechnology , matrix assisted laser desorption/ionization , genotyping , biology , blood culture , chromatography , chemistry , genotype , desorption , virology , genetics , paleontology , organic chemistry , adsorption , gene , antibiotics

Brucella canis was recovered from dogs that were canine brucellosis suspect by blood culture using a modified lysis method. Organism identity was established by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The instrument-provided security library identified the isolates as Brucella species. The isolates were further identified as B. canis with the help of phenotypic and genotypic characteristics. The mass spectral profiles from characterized B. canis isolates, when added to the MALDI-TOF MS standard reference library, allowed successful presumptive identification of B. canis.

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