Open AccessA Comparative Study of an Indirect Fluorescent Antibody Test and an Immunoperoxidase Monolayer Assay for the Diagnosis of Porcine Proliferative Enteropathy
Author(s) -
Roberto Maurício Carvalho Guedes,
Connie J. Gebhart,
N. Winkelman,
Rebecca A.C. Mackie-Nuss
Publication year - 2002
Publication title -
journal of veterinary diagnostic investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.529
H-Index - 78
eISSN - 1943-4936
pISSN - 1040-6387
DOI - 10.1177/104063870201400512
Subject(s) - lawsonia intracellularis , immunoperoxidase , antibody , serology , pathology , immunohistochemistry , direct fluorescent antibody , biology , enteropathy , antigen , medicine , immunology , monoclonal antibody , virology , disease
The currently used indirect fluorescent antibody test (IFAT) for the detection of antibodies against porcine proliferative enteropathy (PPE) was compared to an immunoperoxidase monolayer assay (IPMA). Serum samples used in this comparison were collected from 5-week-old pigs on day 0 (pre-experimental challenge) and on days 7, 14, 21, and 28 after oral inoculation with intestinal homogenate from pigs affected by PPE (28 challenged pigs) and sucrose phosphate glutamate solution (2 control pigs). All animals were euthanized 4 weeks after inoculation. Immunohistochemistry staining was applied to formalin-fixed, paraffin-embedded sections of ileum for the detection of Lawsonia intracellularis antigen. The serology results with each method agreed in all samples, except on days 0 and 7 in 1 control animal, which was positive by IPMA, but negative by IFAT. The percentage of agreement between IFAT and IPMA was 98.6%.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom