SURVIVAL OF ENZYMES IN SECTION FROZEN-SUBSTITUTED TISSUES | Zendy

Jeffrey P. Chang | Zendy; Samuel H. Hori | Zendy

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SURVIVAL OF ENZYMES IN SECTION FROZEN-SUBSTITUTED TISSUES

Author(s) -

Jeffrey P. Chang,

Samuel H. Hori

Publication year - 1962

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/10.5.592

Subject(s) - enzyme , frozen section procedure , chemistry , alkaline phosphatase , adenosine triphosphatase , biochemistry , acid phosphatase , nucleotidase , acetone , nucleotide , atpase , pathology , medicine , gene

Enzyme activities in tissues which have been section frozen-substituted or formol-calcium-fixed have been assayed chemically. The enzymes determined include alkaline and acid phosphatase, adenosine triphosphatase, 5'-nucleotidase, glucose-6-phosphatase, succinic dehydrogenase, as well as di- and triphosphopyridine nucleotide diaphorases. It was found that 22% of glucose-6-phosphatase and more than 70% of the other enzymes assayed could be recovered from the section frozen-substituted tissues. These percentages of recovery are much higher than those obtained in tissues which are fixed in formol-calcium or acetone, or frozen-dried.

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