THE DEMONSTRATION OF THE INDIVIDUAL ACID MUCOPOLYSACCHARIDES IN HUMAN AORTAS, CORONARY ARTERIES AND CEREBRAL ARTERIES. I. THE METHODS | Zendy

Frederick T. Zugibe | Zendy

Open Access

THE DEMONSTRATION OF THE INDIVIDUAL ACID MUCOPOLYSACCHARIDES IN HUMAN AORTAS, CORONARY ARTERIES AND CEREBRAL ARTERIES. I. THE METHODS

Author(s) -

Frederick T. Zugibe

Publication year - 1962

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/10.4.441

Subject(s) - glycosaminoglycan , coronary arteries , hyaluronidase , chondroitin , cerebral arteries , staining , hyaluronic acid , flavobacterium , chondroitin sulfate , chemistry , biochemistry , artery , medicine , enzyme , pathology , anatomy , biology , bacteria , pseudomonas , genetics

A histochemical technique is described for identifying the individual acid mucopolysaccharides in human aortas, coronary arteries and cerebral arteries. Identifications of the various acid mucopolysaccharides depends on treating serial sections with an extract from Flavobacterium heparinum adapted to chondroitin sulfate A, an extract from Flavobacterium adapted to heparitin sulfate, a combination of these two extracts, testicular hyaluronidase, and streptococcal hyaluronidase respectively. Following this treatment and subsequent staining with alcian blue, the identification of the individual acid mucopolysaccharides was made by comparing the treated with the untreated sections. The mechanism of these enzymes is discussed.

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