A HISTOCHEMICAL ANALYSIS OF COENZYME-LINKED DEHYDROGENASES IN SCORBUTIC SEMINAL VESICLES
Author(s) -
Lauro F. Cavazos,
John P. Manning,
J.E. Jeffrey,
W.M. Feagans
Publication year - 1962
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/10.4.387
Subject(s) - biochemistry , ascorbic acid , citric acid cycle , pentose phosphate pathway , succinate dehydrogenase , enzyme , dehydrogenase , biology , vesicle , citric acid , hexose , chemistry , glycolysis , food science , membrane
The effects of ascorbic acid depletion and inanition on the seminal vesicle of the guinea pig were studied histochemically. Through use of the appropriate substrate, several enzymes of the Embden-Meyerhof scheme, the citric acid cycle and the hexose monophosphate shunt were examined. Results indicate that in avitaminosis C, and to a lesser extent in inanition, there is alteration of the functional activity of the cytoplasm of the seminal vesicle epithelium. This change is indicated by decrease of the hexose monophosphate shunt enzymes and, to a limited degree, changes in the citric acid cycle and glutamate dehydrogenase side chain.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom