A METHOD FOR RETAINING BACTERIAL CELLS IN PLACE FOR STAINING AND CYTOCHEMICAL TESTS FOR LIGHT OR ELECTRON MICROSCOPY
Author(s) -
Norman C. Dondero,
M. R. Zelle
Publication year - 1953
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/1.6.415
Subject(s) - electron microscope , staining , microscope slide , microscopy , scanning electron microscope , negative stain , cytochemistry , microscope , optical microscope , cytological techniques , biology , ultrastructure , pathology , microbiology and biotechnology , materials science , optics , anatomy , composite material , medicine , physics
A method of retaining bacterial cells in place on glass cover slips or on electron microscope films for cytochemical tests was described. The method consisted basically of applying a film of "formvar" over the cells on cover slips or on electron microscope object-supporting screens. The "formvar" film did not interfere with many stains and cytochemical tests, nor with the use of enzymes.
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