PRECIPITATION OF CALCIUM PHOSPHATE IN THE HISTOCHEMICAL METHOD FOR PHOSPHATASE | Zendy

George Gömöri | Zendy; Earl P. Benditt | Zendy

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PRECIPITATION OF CALCIUM PHOSPHATE IN THE HISTOCHEMICAL METHOD FOR PHOSPHATASE

Author(s) -

George Gömöri,

Earl P. Benditt

Publication year - 1953

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/1.2.114

Subject(s) - supersaturation , alkaline phosphatase , precipitation , phosphate , chemistry , calcium , phosphatase , diffusion , enzyme , substrate (aquarium) , incubation , biochemistry , inorganic phosphate , chromatography , biophysics , biology , thermodynamics , organic chemistry , ecology , physics , meteorology

Ca phosphate, when precipitated by the addition of phosphate to solutions similar in composition to the histochemical substrate mixture, does not show any experimentally demonstrable tendency to supersaturation. The factors which may contribute to false localizations of enzymatic activity its the Ca-CoS method for alkaline phosphatase are not well understood and certainly not amenable to mathematical analysis for the time being. At present, diffusion artifacts do not appear to be an important source of error, provided incubation time is not unduly extended.

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