Open AccessStamina-Enhancing Effects of Human Adipose-Derived Stem Cells
Author(s) -
EunJung Yoon,
Hye Rim Seong,
Jangbeen Kyung,
Dajeong Kim,
Sangryong Park,
EhnKyoung Choi,
Yun-Bae Kim,
Dongsun Park
Publication year - 2021
Publication title -
cell transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.043
H-Index - 100
eISSN - 1555-3892
pISSN - 0963-6897
DOI - 10.1177/09636897211035409
Subject(s) - lactate dehydrogenase , medicine , adipose tissue , endocrinology , glycogen , neurotrophic factors , transplantation , brain derived neurotrophic factor , aspartate transaminase , creatine kinase , stem cell , chemistry , biology , biochemistry , microbiology and biotechnology , enzyme , alkaline phosphatase , receptor
Stamina-enhancing effects of human adipose derived stem cells (hADSCs) were investigated in young Sprague-Dawley rats. Ten-day-old male rats were transplanted intravenously (IV) or intracerebroventricularly (ICV) with hADSCs (1 × 10 6 cells/rat), and physical activity was measured by locomotor activity and rota-rod performance at post-natal day (PND) 14, 20, 30, and 40, as well as a forced swimming test at PND 41. hADSCs injection increased the moving time in locomotor activity, the latency in rota-rod performance, and the maximum swimming time. For the improvement of physical activity, ICV transplantation was superior to IV injection. In biochemical analyses, ICV transplantation of hADSCs markedly reduced serum creatine phosphokinase, lactate dehydrogenase, alanine transaminase, and muscular lipid peroxidation, the markers for muscular and hepatic injuries, despite the reduction in muscular glycogen and serum triglycerides as energy sources. Notably, hADSCs secreted brain-derived neurotrophic factor (BDNF) and nerve growth factor in vitro, and increased the level of BDNF in the brain and muscles in vivo. The results indicate that hADSCs enhance physical activity including stamina not only by attenuating tissue injury, but also by strengthening the muscles via production of BDNF.