Open AccessA comprehensive gene expression profile of allergic rhinitis-derived nasal fibroblasts and the potential mechanism for its phenotype
Author(s) -
Zhengwen Li,
Wentao Zou,
Jingwen Sun,
Shuang Zhou,
Yue Zhou,
Xiaoyu Cai,
Jiaxiong Zhang
Publication year - 2022
Publication title -
human and experimental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 78
eISSN - 1477-0903
pISSN - 0960-3271
DOI - 10.1177/09603271211069038
Subject(s) - gene silencing , downregulation and upregulation , transcription factor , biology , gene , immune system , gene expression , phenotype , inflammation , cytokine , fold change , immunology , microbiology and biotechnology , genetics
Background Allergic rhinitis (AR) is a common immunoglobulin E-mediated immune response involved various cell types, while the role of nasal fibroblasts (NFs) in the pathogenesis of AR is less understood.Purpose The study aimed to uncover the gene expression profile of AR-derived NFs and the potential mechanism for the changed phenotype of AR-NFs.Research Design The primary NFs were isolated from 3 AR patients (AR-NFs) and 3 controls (Ctrl-NFs), and the proliferation, migration and interleukins production abilities of NFs were detected respectively. RNA-sequence was used to identify differentially expressed genes (DEGs) in AR-NFs. Transcription factor (TF) regulatory network and bioinformatic analyses were both conducted to clarify the biological roles of DEGs including the TFs. The DEG with the highest validated |fold change (FC)| value, detected by qPCR, was selected for further confirmation.Results AR-NFs showed a higher proliferation and migration abilities as well as released higher levels of IL-33 and IL-6, compared to Ctrl-NFs. A total of 729 DEGs were screened out in AR-NFs. TF regulatory network indicated that BARX homeobox 1 (BARX1) and forkhead box L1 were the major node TFs. Bioinformatic analyses showed that a large number of DEGs including several target genes of BARX1 were both enriched cytokine-related GO terms, and immune- or inflammation-related pathways. BARX1 had the highest |FC| value, and silencing BARX1 in AR-NFs resulted in the significant downregulation of proliferation and migration abilities, and the production of interleukins.Conclusions Our study for the first time provided the gene expression profile of AR-derived NFs, and BARX1 could be developed as a potent target to alleviate the pathogenesis of AR.