Open AccessIn vitro Activity of a Novel Series of Polyoxosilicotungstates against Human Myxo-, Herpes- and Retroviruses
Author(s) -
Satoru Ikeda,
Johan Neyts,
Naohiko Yamamoto,
Barry A. Murrer,
Brian Theobald,
Gerald E. Bossard,
Geoffrey Henson,
Michael J. Abrams,
D. Picker,
Erik De Clercq
Publication year - 1993
Publication title -
antiviral chemistry and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.919
H-Index - 51
eISSN - 2040-2066
pISSN - 0956-3202
DOI - 10.1177/095632029300400501
Subject(s) - herpes simplex virus , virus , virology , human cytomegalovirus , biology , in vitro , chemistry , biochemistry
A series of silicon-containing polyoxotungstates belonging to the ‘Keggin-type’ (‘Keggin’, ‘Keggin sandwich’) were evaluated for their antiviral activity against enveloped viruses (myxo-, herpes- and retroviruses). The compounds exhibited antiviral activity against influenza virus type A, respiratory syncytial virus (RSV), herpes simplex virus type-1 (HSV-1), type-2 (HSV-2), thymidine kinase-deficient (TIC) HSV-1, human cytomegalovirus (HCMV), human immunodeficiency virus type-1 (HIV-1) and type-2 (HIV-2) at concentrations that were well below their cytotoxic threshold. The ‘Keggin’ compound JM2815 (K 5 [Si-(TiCp)W 11 O 39 ].12H 2 O) and the ‘Keggin sandwich’ compound JM1590 (K 13 [Ce(SiW 11 O 39 ) 2 ].26H 2 O) resulted in the highest selectivity indices against HIV-1 and HIV-2, and compound JM2820 ([Me 3 NH] 8 .[Si 2 Nb 6 W 18 O 77 ]) was the most potent inhibitor of HSV and HCMV replication. These compounds proved active against HCMV and HSV when present during virus adsorption, and against influenza virus A and RSV when present after virus adsorption. Polyoxosilicotungstates inhibited the binding of radiolabeled HCMV particles to the cells at concentrations that were antivirally active, and the compounds were able to displace HCMV particles that were bound to a heparin-Sepharose matrix. Presumably, the polyoxosilicotungstates interact with positively charged domains on the viral envelope site(s) involved in the attachment of the (HCMV) virions to the cell surface receptor heparan sulphate.