
Morphostructural Analysis of Human Follicular Stem Cells on Highly Porous Bone Hydroxyapatite Scaffold
Author(s) -
Stefano Tetè,
Filiberto Mastrangelo,
Luigi Carone,
Eleonora Nargi,
G. Costanzo,
Raffaele Vinci,
F Burruano,
Silvia Tortorici,
V Dadorante,
Francesco Caciagli,
Tonino Traini,
Enrico Gherlone,
Auro Caraffa,
Vincenzo Salini,
Pio Conti,
Renata Ciccarelli
Publication year - 2007
Publication title -
international journal of immunopathology and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.724
H-Index - 53
eISSN - 2058-7384
pISSN - 0394-6320
DOI - 10.1177/039463200702000418
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , scaffold , extracellular matrix , stem cell , cd90 , cd146 , osteoblast , chemistry , matrix (chemical analysis) , biomedical engineering , materials science , in vitro , biology , cd34 , medicine , biochemistry , chromatography
In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3–6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss®). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the culture of mesenchymal stem cells derived from adult bone marrow. Also, after osteogenic differentiation, these analyses indicate typical osteoblast morphostructure and reveale a tri-dimensional organization of the cells and deposition of extracellular matrix (ECM) in close contact with biomaterial. This approach would allow to personalize the scaffold for bone tissue engineering in order to accelerate the process of osteogenesis.