Open AccessNox2 underpins microvascular inflammation and vascular contributions to cognitive decline
Author(s) -
Alessio Alfieri,
Juraj Koudelka,
Mosi Li,
Sanny Scheffer,
Jessica Duncombe,
Andrea Caporali,
Rajesh N. Kalaria,
Colin Smith,
Ajay Shah,
Karen Horsburgh
Publication year - 2022
Publication title -
journal of cerebral blood flow and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.167
H-Index - 193
eISSN - 1559-7016
pISSN - 0271-678X
DOI - 10.1177/0271678x221077766
Subject(s) - inflammation , nicotinamide adenine dinucleotide phosphate , nadph oxidase , cognitive decline , oxidative stress , medicine , vascular disease , pathology , reactive oxygen species , white matter , endocrinology , biology , dementia , disease , microbiology and biotechnology , magnetic resonance imaging , oxidase test , biochemistry , radiology , enzyme
Chronic microvascular inflammation and oxidative stress are inter-related mechanisms underpinning white matter disease and vascular cognitive impairment (VCI). A proposed mediator is nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (Nox2), a major source of reactive oxygen species (ROS) in the brain. To assess the role of Nox2 in VCI, we studied a tractable model with white matter pathology and cognitive impairment induced by bilateral carotid artery stenosis (BCAS). Mice with genetic deletion of Nox2 (Nox2 KO) were compared to wild-type (WT) following BCAS. Sustained BCAS over 12 weeks in WT mice induced Nox2 expression, indices of microvascular inflammation and oxidative damage, along with white matter pathology culminating in a marked cognitive impairment, which were all protected by Nox2 genetic deletion. Neurovascular coupling was impaired in WT mice post-BCAS and restored in Nox2 KO mice. Increased vascular expression of chemoattractant mediators, cell-adhesion molecules and endothelial activation factors in WT mice post-BCAS were ameliorated by Nox2 deficiency. The clinical relevance was confirmed by increased vascular Nox2 and indices of microvascular inflammation in human post-mortem subjects with cerebral vascular disease. Our results support Nox2 activity as a critical determinant of VCI, whose targeting may be of therapeutic benefit in cerebral vascular disease.