Allergen Extract Compatibilities

Objective 1) Assess the relative stabilities of extracts at various dilutions and define target allergen recoveries in defined mixtures. 2) Identify stable, risky, and incompatible extract combinations. 3) Recognize the effects of glycerin, time to and temperature on the stability of immunotherapy treatment vials. 4) Design optimal extract mixtures. Method Examination of target allergen recoveries in extract mixtures at maintenance vial strengths, using laboratory methods (IgE ELISA inhibitor and IgE Immunoblotting) known to be sensitive to changes in overall extract composition, specific allergen concentrations, and total allergen concentrations or extract potencies, 2000 present. Results Degradation of some allergens in dilutions or mixtures can be influenced by several factors, including endogenous hydrolytic enzymes, final glycerin concentrations, presence of stabilizers such as human serum albumin, storage times in the refrigerator, and elevated temperature exposures. A working knowledge of the stabilities and compatibilities of various allergens in extract mixtures is essential to optimal formulation of patient vials for subcutaneous immunotherapy. A mixing recommendation chart was created for use in the allergy clinic as a guide to ensure allergen extract compatibility. Conclusion Degradation of allergens in dilutions or mixtures can be influenced by several factors, including presence of endogenous hydrolytic enzymes, final glycerin concentrations, presence of stabilizers such as human serum albumin, storage refrigerator times, and elevated temperature exposures.