Open AccessRole of Prostaglandin D2 Receptor in Otitis Media
Author(s) -
Kariya Shin,
Okano Mitsuhiro,
Eguchii Motocharu,
Makihara Seiichiro,
Higaki Takaya,
Nishizaki Kazunori,
Morita Norimasa
Publication year - 2011
Publication title -
otolaryngology–head and neck surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.232
H-Index - 121
eISSN - 1097-6817
pISSN - 0194-5998
DOI - 10.1177/0194599811415823a301
Subject(s) - otitis , lipopolysaccharide , prostaglandin d2 , receptor , immunology , chemokine , prostaglandin , chemotaxis , prostaglandin e2 receptor , medicine , endocrinology , chemistry , surgery , agonist
Objective Prostaglandin D2 is the major prostanoid involved in infection and allergy. D prostanoid receptor (DP) and chemoattractant receptor‐homologous molecule expressed on T helper type 2 (Th2) cells (CRTH2) are major prostaglandin D2 receptors. The purpose of this study is to disclose the role of prostaglandin D2 receptors in otitis media. Method Experimental otitis media was induced by middle ear injection of lipopolysaccharide. DP single gene‐decient (DP‐/‐) mice, CRTH2 single gene‐decient (CRTH2‐/‐) mice, and DP/CRTH2 double gene‐decient (DP‐/‐ CRTH2‐/‐) mice also received lipopolysaccharide. Concentrations of cytokines in middle ear effusion were measured by ELISA. Results Signicant production of cytokines and chemokines was detected in lipopolysaccharide‐treated wild‐type mice. CRTH2‐/‐ mice also showed significant up regulation of inflammatory reactions. In contrast to CRTH2‐/‐ mice, inltration of inammatory cells and expression of macrophage inammatory protein 2, IL‐1‐beta, and IL‐6 induced by lipopolysaccharide were reduced signicantly in DP‐/‐ mice and DP‐/‐CRTH2‐/‐ mice. Conclusion These findings indicate that DP may play significant roles in pathogenesis of experimental otitis media.