Open AccessMethylome Analysis of HPV‐Associated Head and Neck Cancer
Author(s) -
Hernandez Maria Ivette Mucino,
Ojeda Alejandro Gonzalez,
Alvarea Alfonso Bencomo,
Jeahns Elias Morales,
Camacho Jose Gonzalo Vazquez,
Rodriguez Luz Margarita Baltazar,
Fuentes Hector Montoya
Publication year - 2011
Publication title -
otolaryngology–head and neck surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.232
H-Index - 121
eISSN - 1097-6817
pISSN - 0194-5998
DOI - 10.1177/0194599811415823a122
Subject(s) - thyroid cancer , pathology , medicine , polymerase chain reaction , thyroid , restriction fragment length polymorphism , hyperplasia , population , biology , gene , genetics , environmental health
Objective Estimate the HPV frequency and types in neoplastic thyroid tissue by means of Polymerase Chain Reaction‐Restriction Fragments Length Polymorphisms (PCR‐RFLP). Method Transversal study. We analyzed 157 samples from malignant and benign thyroid tumors of paraffin‐embedded tissue. All the paraffin blocks were randomly chosen from the archive of Pathology Tissue Bank (Centro Medico Nacional de Occidente, IMSS). The molecular detection and typing was performed at Molecular Microbiology II Laboratory in CIBO. Results The HPV observed frequency was 2.5% (4 cases). HPV‐6 was found in 2 cases of thyroid hyperplasia (2.5%) and HPV‐33 in 2 cases of Papillary Cancer (4.6%). Conclusion HPV is not frequent in thyroid neoplasms, at least in the studied population. The HPV types found were HPV‐6 in hyperplasia and HPV‐33 in papillary cancer.