Open AccessCalcium-activated Chloride Channel-2 in Human Epithelia
Author(s) -
Che J. Con,
Kenta Yamasaki,
Satoshi Kawasaki,
Andrew J. Quantock,
Noriko Koizumi,
Shigeru Kinoshita
Publication year - 2004
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/002215540405200313
Subject(s) - basement membrane , chloride channel , calcium , microbiology and biotechnology , epithelium , chemistry , cytosol , biology , biophysics , biochemistry , enzyme , organic chemistry , genetics
Calcium-activated chloride channels (CLCAs) are a family of multifunctional proteins that are widely distributed in tissues. To investigate the distribution of human CLCA-2 (hCLCA2) in human epithelia at the light and electron microscopic levels, we raised a primary antibody against a synthetic polypeptide sequence from natural hCLCA2. Corneal, skin, vaginal, esophageal, and laryngeal epithelia were immunopositive for hCLCA2 at the cytosolic aspect of the basal cells adjacent to the basement membrane. Epithelia of stomach and small intestine showed no hCLCA2 immunoreactivity. This study reports the cellular distribution of hCLCA2 in human epithelia and suggests its possible involvement in epithelial stratification and cell-substrate adhesion.
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