Open AccessCellular Localization of Group IIA Phospholipase A2 in Rats
Author(s) -
Toshiyuki Yoshikawa,
Satoru Naruse,
Motoji Kitagawa,
Hiroshi Ishiguro,
Masato Nagahama,
Etsuko Yasuda,
Reiji Semba,
Minoru Tanaka,
Kohji Nomura,
Tetsuo Hayakawa
Publication year - 2001
Publication title -
journal of histochemistry and cytochemistry/the journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/002215540104900611
Subject(s) - spleen , in situ hybridization , messenger rna , biology , microbiology and biotechnology , kidney , antibody , enzyme , medicine , endocrinology , immunology , biochemistry , gene
It has been known that group II phospholipase A 2 (PLA 2 ) mRNA and protein are present in the homogenates of the spleen, lung, liver, and kidney in normal rats, but the cellular origin of this enzyme has not been yet identified. At present, five subtypes of group II PLA 2 have been identified in mammals. Antibodies or mRNA probes previously used for detecting group II PLA 2 need to be evaluated to identify the subtypes of group II PLA 2 . In this study we tried to identify group IIA PLA 2 -producing cells in normal rat tissues by in situ hybridization (ISH) using an almost full-length RNA probe for rat group IIA enzyme. Group IIA PLA 2 mRNA was detected in megakaryocytes in the spleen and Paneth cells in the intestine by ISH. These cells were also immunopositive for an antibody raised against group IIA PLA 2 isolated from rat platelets. Group IIA PLA 2 mRNA-positive cells were not detected in lung, liver, kidney, and pancreas. Under normal conditions, group IIA PLA 2 -producing cells are splenic megakaryocytes and intestinal Paneth cells in rats.