Open AccessNovel Instrumentation for Real-Time Monitoring Using Miniaturized Flow Systems with Integrated Biosensors
Author(s) -
R. Freaney,
A. J. McShane,
T. V. Keaveny,
Malachi J. McKenna,
Kai Rabenstein,
Frieder W. Scheller,
D. Pfeiffer,
G. Urban,
I. Moser,
G. Jobst,
Andreas Manz,
Elisabeth Verpoorte,
Mirjam Widmer,
Dermot Diamond,
Eithne Dempsey,
F. J. Sáez de Viteri,
Malcolm R. Smyth
Publication year - 1997
Publication title -
annals of clinical biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.6
H-Index - 80
eISSN - 1758-1001
pISSN - 0004-5632
DOI - 10.1177/000456329703400312
Subject(s) - microdialysis , biosensor , biomedical engineering , continuous glucose monitoring , peristaltic pump , instrumentation (computer programming) , chromatography , chemistry , computer science , engineering , biochemistry , biology , extracellular , operating system , insulin , endocrinology , mechanical engineering , glycemic
A prototype miniaturized Total Chemical Analysis System (μTAS) has been developed and applied to on-line monitoring of glucose and lactate in the core blood of anaesthetized dogs. The system consists of a highly efficient microdialysis sampling interface sited in a small-scale extracorporeal shunt circuit (‘MiniShunt’), a silicon machined microflow manifold and integrated biosensor array for glucose and lactate detection with associated computer software for analytical process control. During in-vivo testing the device allowed real-time on-screen monitoring of glucose and lactate with system response times of less than 5 min, made possible by the small dead volume of the microflow system. On-line glucose and lactate measurements were made in the basal state as well as during intravenous infusion of glucose or lactate. The prototype μTAS is currently suitable for trend monitoring but refinements are necessary before application of the system for determination of individual lactate values.