Impact of Vital Dyes on Cell Viability and Transduction Efficiency of AAV Vectors Used in Retinal Gene Therapy Surgery: An In Vitro and In Vivo Analysis | Zendy

Anna Paola Salvetti | Zendy; Maria I. Patrício | Zendy; Alun R. Barnard | Zendy; Harry O. Orlans | Zendy; Doron Hickey | Zendy; Robert E. MacLaren | Zendy

Open Access

Impact of Vital Dyes on Cell Viability and Transduction Efficiency of AAV Vectors Used in Retinal Gene Therapy Surgery: An In Vitro and In Vivo Analysis

Author(s) -

Anna Paola Salvetti,

Maria I. Patrício,

Alun R. Barnard,

Harry O. Orlans,

Doron Hickey,

Robert E. MacLaren

Publication year - 2017

Publication title -

translational vision science and technology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.508

H-Index - 21

ISSN - 2164-2591

DOI - 10.1167/tvst.6.4.4

Subject(s) - genetic enhancement , viability assay , retinal , transduction (biophysics) , in vivo , in vitro , microbiology and biotechnology , cell therapy , medicine , chemistry , biology , computational biology , gene , ophthalmology , biochemistry , stem cell

This paper might be of interest to the retinal gene therapy community: it is a "bench to bedside" research paper about the potential use of dyes as a surgical adjunct during the gene therapy surgery. We have tested the potential toxicity and impact on transduction efficiency in an in vitro and in vivo model.

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