Viability of Mouse Retinal Explant Cultures Assessed by Preservation of Functionality and Morphology | Zendy

Virpi Alarautalahti | Zendy; Symantas Ragauskas | Zendy; Jenni J. Hakkarainen | Zendy; Hannele UusitaloJärvinen | Zendy; Hannu Uusitalo | Zendy; Jari Hyttinen | Zendy; Giedrius Kalesnykas | Zendy; Soile Nymark | Zendy

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Viability of Mouse Retinal Explant Cultures Assessed by Preservation of Functionality and Morphology

Author(s) -

Virpi Alarautalahti,

Symantas Ragauskas,

Jenni J. Hakkarainen,

Hannele UusitaloJärvinen,

Hannu Uusitalo,

Jari Hyttinen,

Giedrius Kalesnykas,

Soile Nymark

Publication year - 2019

Publication title -

investigative ophthalmology and visual science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.935

H-Index - 218

eISSN - 1552-5783

pISSN - 0146-0404

DOI - 10.1167/iovs.18-25156

Subject(s) - retinal , explant culture , retina , biology , gliosis , microbiology and biotechnology , muller glia , viability assay , neuroscience , in vitro , stem cell , botany , progenitor cell , biochemistry

Retinal explant cultures provide simplified systems where the functions of the retina and the effects of ocular therapies can be studied in an isolated environment. The purpose of this study was to provide insight into long-term preservation of retinal tissue in culture conditions, enable a deeper understanding of the interdependence of retinal morphology and function, and ensure the reliability of the explant technique for prolonged experiments.

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