Microarray Characterization of Human Embryonic Stem Cell–Derived Retinal Cultures | Zendy

Deepak A. Lamba | Zendy; Thomas A. Reh | Zendy

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Microarray Characterization of Human Embryonic Stem Cell–Derived Retinal Cultures

Author(s) -

Deepak A. Lamba,

Thomas A. Reh

Publication year - 2011

Publication title -

investigative ophthalmology and visual science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.935

H-Index - 218

eISSN - 1552-5783

pISSN - 0146-0404

DOI - 10.1167/iovs.10-6504

Subject(s) - retinal , retina , biology , embryonic stem cell , retinal pigment epithelium , transcriptome , microarray analysis techniques , gene expression profiling , muller glia , induced pluripotent stem cell , microarray , stem cell , microbiology and biotechnology , gene expression , genetics , gene , neuroscience , progenitor cell , biochemistry

A number of protocols have been published to induce retinal determination from human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC). Although all these studies have shown some degree of expression of markers of retinal cells, fewer than 30 markers are typically used to characterize the ESC-derived retinal cells. Hence, it is not known whether they express all the genes present in normal developing retinal cells. To assess the efficiency of their retinal determination protocol at the transcriptome level and to understand the changes in human retinal gene expression patterns during development, the authors conducted a microarray-based analysis comparing human retina to hESC-derived retinal cells.

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