Open AccessConfluent Monolayers of Cultured Human Fetal Retinal Pigment Epithelium Exhibit Morphology and Physiology of Native Tissue
Author(s) -
Arvydas Maminishkis,
Shan Chen,
S. Jalickee,
Tina Banzon,
Guangpu Shi,
Fei E. Wang,
Todd Ehalt,
Jeffrey A. Hammer,
Sheldon S. Miller
Publication year - 2006
Publication title -
investigative ophthalmology and visual science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.935
H-Index - 218
eISSN - 1552-5783
pISSN - 0146-0404
DOI - 10.1167/iovs.05-1622
Subject(s) - retinal pigment epithelium , biology , tight junction , electron microscope , epithelium , retinal , microbiology and biotechnology , chemistry , anatomy , biochemistry , physics , genetics , optics
Provide a reproducible method for culturing confluent monolayers of hfRPE cells that exhibit morphology, physiology, polarity, and protein expression patterns similar to native tissue.